This function counts the number of reads for each barcode in each sample. It is a quality control function (QC) to see if the biological protocol went as planned. If a sample has very low mapped compared to the other means that is has a lower quality.
Arguments
- screenR_Object
The ScreenR object obtained using the
create_screenr_object
Examples
object <- get0("object", envir = asNamespace("ScreenR"))
head(count_mapped_reads(object))
#> # A tibble: 6 × 3
#> Barcode Sample Mapped
#> <fct> <fct> <int>
#> 1 10208 T1 3520
#> 2 10502 T1 280
#> 3 10796 T1 1507
#> 4 11089 T1 3797
#> 5 11382 T1 717
#> 6 11675 T1 7330